Basic Information:

  VIRBase ID:  

VHID00256536

  Virus:  

Human betaherpesvirus 5 (HHV-5)

  Host:  

Homo sapiens

  Confidence Score:  

0.9374

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-UL112-5p:  MIMAT0026552          VAMP3:  NM_004781     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-UL112-5p	VAMP3
miRBase Accession/Entrez ID	MIMAT0026552	9341
Organism	Human betaherpesvirus 5 (HHV-5)	Homo sapiens
Category	miRNA	mRNA
Alias	-	CEB

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	VAMP3	chr1:7836923(+) A-I      intronic chr1:7836917(+) A-I      intronic chr1:7836926(+) A-I      intronic chr1:7832888(+) A-I      ncRNA chr1:7832894(+) A-I      ncRNA chr1:7832856(+) A-I      ncRNA chr1:7832829(+) A-I      ncRNA chr1:7832828(+) A-I      ncRNA chr1:7832802(+) A-I      ncRNA chr1:7836930(+) A-I      intronic chr1:7832794(+) A-I      ncRNA chr1:7839230(+) A-I      intronic chr1:7835681(+) A-I      intronic chr1:7837133(+) A-I      intronic chr1:7836984(+) A-I      intronic chr1:7837132(+) A-I      intronic chr1:7837114(+) A-I      intronic chr1:7837105(+) A-I      intronic chr1:7832759(+) A-I      intronic chr1:7832699(+) A-I      intronic chr1:7837006(+) A-I      intronic chr1:7832784(+) A-I      ncRNA chr1:7836910(+) A-I      intronic chr1:7836898(+) A-I      intronic chr1:7837060(+) A-I      intronic chr1:7836947(+) A-I      intronic chr1:7837009(+) A-I      intronic chr1:7837139(+) A-I      intronic chr1:7837080(+) A-I      intronic chr1:7839242(+) A-I      intronic chr1:7837049(+) A-I      intronic chr1:7835671(+) A-I      intronic chr1:7832693(+) A-I      intronic chr1:7837145(+) A-I      intronic

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	VAMP3	chr1:7832772-7832773(+) m6A 5'UTR//intron       24284625//24981863 chr1:7832846-7832847(+) m6A 5'UTR//intron       24284625//25456834//24981863//22608085 chr1:7832869-7832870(+) m6A 5'UTR//intron       24284625//25456834//24981863//22608085 chr1:7832939-7832940(+) m6A 5'UTR//intron       24284625//24209618//25456834//24981863//22608085 chr1:7833127-7833128(+) m6A 5'UTR//intron       24284625//24209618//24981863//22608085 chr1:7833194-7833195(+) m6A 5'UTR//intron       24284625//24981863//22608085 chr1:7833211-7833212(+) m6A 5'UTR//intron       24284625//24981863//22608085 chr1:7833540-7833541(+) m6A 5'UTR//CDS       24981863 chr1:7833550-7833551(+) m6A 5'UTR//CDS       24981863 chr1:7837226-7837227(+) m6A 5'UTR//CDS//exon       24981863 chr1:7837247-7837248(+) m6A CDS//exon       24981863 chr1:7840046-7840047(+) m6A 3'UTR       26404942 chr1:7840058-7840059(+) m6A 3'UTR       26404942 chr1:7840102-7840103(+) m6A 3'UTR       26404942 chr1:7840896-7840897(+) m6A 3'UTR       24981863//27773535 chr1:7840957-7840958(+) m6A 3'UTR       24981863//27773535 chr1:7840974-7840975(+) m6A 3'UTR       24981863//27773535 chr1:7840994-7840995(+) m6A 3'UTR       24981863//27773535 chr1:7841046-7841047(+) m6A 3'UTR       24284625//24981863//27773535//22608085 chr1:7841074-7841075(+) m6A 3'UTR       24284625//24981863//27773535//22608085 chr1:7841080-7841081(+) m6A 3'UTR       24284625//24981863//27773535//22608085 chr1:7841122-7841123(+) m6A 3'UTR       24284625//24981863//27773535//22608085 chr1:7841179-7841180(+) m6A 3'UTR       24284625//24981863//22608085 chr1:7841295-7841296(+) m6A 3'UTR       24284625 chr1:7841344-7841345(+) m6A 3'UTR       24284625 chr1:7841378-7841379(+) m6A 3'UTR       24284625 chr1:7841448-7841449(+) m6A 3'UTR       24284625

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	VAMP3	Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)     - Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleus HeLa-S3 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-UL112-5p
Interactor2: VAMP3

Evidence Support:

Strong-Evidence	Luciferase reporter assay//Western blot
Weak-Evidence	RIP-Chip
Support Database	VmiReg

References:

PMID	24629342	Target region	None
Source	VmiReg	Interactor1 expression	None
Tissue or cell line	293T cells	Interactor2 expression	None
Description	We find that HCMV miRs UL112-1,US5-1, and US5-2 target multiple components of the host secretory pathway, including VAMP3, RAB5C, RAB11A, SNAP23, and CDC42.