Basic Information:

  VIRBase ID:  

VHID00232483

  Virus:  

Human betaherpesvirus 5 (HHV-5)

  Host:  

Homo sapiens

  Confidence Score:  

0.9860

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-2-3p:  MIMAT0001583          EIF4A1:  NM_001416     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-2-3p	EIF4A1
miRBase Accession/Entrez ID	MIMAT0001583	1973
Organism	Human betaherpesvirus 5 (HHV-5)	Homo sapiens
Category	miRNA	mRNA
Alias	-	DDX2A//EIF-4A//eIF-4A-I//EIF4A//eIF4A-I

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	EIF4A1	chr17:7479492(+) A-I      intronic

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	EIF4A1	chr17:7476026-7476027(+) m6A 5'UTR       24981863 chr17:7476079-7476080(+) m6A 5'UTR       24981863 chr17:7476660-7476661(+) m6A 5'UTR//intron       24284625//25456834//24981863//22575960 chr17:7476818-7476819(+) m6A 5'UTR//CDS//exon//intron       - chr17:7477551-7477552(+) m6A 5'UTR//intron       25456834//24981863 chr17:7477556-7477557(+) m6A 5'UTR//intron       25456834//24981863 chr17:7477569-7477570(+) m6A 5'UTR//intron       25456834//24981863 chr17:7477585-7477586(+) m6A 5'UTR//CDS//exon//intron       25456834//24981863//26404942//27773535 chr17:7477631-7477632(+) m6A exon//intron       25456834//24981863 chr17:7477903-7477904(+) m6A 3'UTR//CDS//exon//intron       - chr17:7478146-7478147(+) Tm exon//intron       - chr17:7479291-7479292(+) m6A exon//intron       - chr17:7479314-7479315(+) m6A exon//intron       - chr17:7479869-7479870(+) m6A 3'UTR//CDS//exon//intron       22575960 chr17:7479933-7479934(+) m6A 3'UTR//CDS//exon//intron       27773535//22575960 chr17:7480180-7480181(+) Tm exon//intron       - chr17:7480439-7480440(+) m6A 3'UTR//CDS//exon       27773535//22575960 chr17:7480725-7480726(+) m6A 3'UTR//CDS//exon//intron       27773535//22575960 chr17:7480899-7480900(+) m6A 3'UTR//CDS//exon       27773535//22575960 chr17:7480922-7480923(+) m6A 3'UTR//CDS//exon       27773535//22575960 chr17:7480974-7480975(+) m6A 3'UTR//CDS//exon       26404942//27773535//22575960 chr17:7481098-7481099(+) m6A 3'UTR//exon//intron       26404942//27773535//22575960 chr17:7481157-7481158(+) m6A 3'UTR//CDS//exon       26404942//27773535//22575960 chr17:7481247-7481248(+) m6A 3'UTR//exon//intron       26404942//27773535//22575960 chr17:7481299-7481300(+) m6A 3'UTR//exon//intron       27773535 chr17:7481375-7481376(+) Tm 3'UTR//exon//intron       - chr17:7481549-7481550(+) m6A 3'UTR//CDS//exon//intron       27773535//22575960 chr17:7481579-7481580(+) m6A exon//intron       27773535 chr17:7481721-7481722(+) m6A 3'UTR//CDS//exon       27773535//22575960 chr17:7481729-7481730(+) m6A 3'UTR//CDS//exon       27773535//22575960 chr17:7481739-7481740(+) m6A 3'UTR//CDS//exon       27773535//22575960 chr17:7481747-7481748(+) m6A 3'UTR//CDS//exon       27773535//22575960 chr17:7481895-7481896(+) m6A 3'UTR//exon       24981863//27773535//22575960 chr17:7481951-7481952(+) m6A 3'UTR       24981863//27773535 chr17:7481958-7481959(+) m6A 3'UTR       24981863//27773535 chr17:7481967-7481968(+) m6A 3'UTR       24981863//27773535 chr17:7482033-7482034(+) m6A 3'UTR       27773535 chr17:7482121-7482122(+) m6A 3'UTR       27773535//22575960 chr17:7482148-7482149(+) m6A 3'UTR       27773535//22575960 chr17:7482166-7482167(+) m6A 3'UTR       27773535//22575960 chr17:7482175-7482176(+) m6A 3'UTR       27773535//22575960 chr17:7482284-7482285(+) m6A 3'UTR       27773535 chr17:7482318-7482319(+) m6A 3'UTR       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	EIF4A1	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)|HeLa-S3 cells     - Exosome Blood     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     -

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID
RNAactDrug	hcmv-miR-US25-2-3p	Irinotecan       60838

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-2-3p
Interactor2: EIF4A1

Evidence Support:

Strong-Evidence	Hybrid-PCR//Luciferase reporter assay//qRT-PCR//Western blot
Prediction-Evidence	miRanda//RNAHybrid
Support Database	ViRBase

References:

PMID	23747307	Target region	3'UTR
Source	ViRBase	Interactor1 expression	Upregulation
Tissue or cell line	MRC-5 cells	Interactor2 expression	Downregulation
Description	In our study, eukaryotic translation initiation factor 4A1 (eIF4A1) was identified to be a direct target of miR-US25-2-3p. Small interfering RNA (siRNA) and miR-US25-2-3p mediated eIF4A1 knockdown experiments revealed that high level of miR-US25-2-3p in MRC-5 cells decreased HCMV and host genomic DNA synthesis, and inhibited cap-dependent translation and host cell proliferation. However, eIF4A1 up-regulation induced by miR-US25-2-3p inhibitor increased HCMV copy number.