Detail Information

Basic Information:


  VIRBase ID:  

VHID00224168

  Virus:  

Human betaherpesvirus 5 (HHV-5)

  Host:  

Homo sapiens

  Confidence Score:  

0.9865

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-UL148D:           IER3:      

      *It may take a few minutes to display results.

Interactor Information:


Interactor1 Interactor2
Symbol hcmv-miR-UL148D IER3
miRBase
Accession/Entrez ID		 MIMAT0001578 8870
Organism Human betaherpesvirus 5 (HHV-5) Homo sapiens
Category miRNA mRNA
Alias - DIF-2//DIF2//GLY96//IEX-1//IEX-1L//IEX1//PRG1

RNA Modification:


Resource Symbol ModificationPosition Type Genomic Context PMID
RMBase IER3
chr6:30711003-30711004(-) m6A 3'UTR       24284625//27773535
chr6:30711027-30711028(-) Gm 3'UTR       -
chr6:30711135-30711136(-) m6A 3'UTR       24284625//24209618//27773535
chr6:30711145-30711146(-) m6A 3'UTR       24284625//24209618//27773535
chr6:30711255-30711256(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085
chr6:30711287-30711288(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085
chr6:30711296-30711297(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085
chr6:30711311-30711312(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//22575960//22608085
chr6:30711525-30711526(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085
chr6:30711541-30711542(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085
chr6:30711557-30711558(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085
chr6:30711595-30711596(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085
chr6:30711602-30711603(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085
chr6:30711620-30711621(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085
chr6:30711663-30711664(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085
chr6:30711674-30711675(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085
chr6:30711752-30711753(-) m6A 3'UTR//CDS       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085
chr6:30711764-30711765(-) m6A 3'UTR//CDS       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085
chr6:30711941-30711942(-) m6A CDS       24284625//24209618//27773535//22575960
chr6:30711946-30711947(-) m6A CDS       24284625//24209618//27773535//22575960
chr6:30712217-30712218(-) m6A CDS       24284625//25456834//24981863//27371828//22575960

RNA Localization:


Resource Symbol Subcellular Localization Tissue or Cell Line PMID
RNALocate hcmv-miR-UL148D
Exosome K562 cells     24460325
IER3
Cytosol HCC cell line (HepG2)     -
Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539
Exosome Blood     -
Ribosome-free cytosol Colon cancer cells     24393600

Related Drug Information:


Resource Symbol Drug Name PubChem ID
RNAactDrug hcmv-miR-UL148D
Crizotinib       11626560

Interaction Network (The top 100 interactions):


Interactor1: hcmv-miR-UL148D
Interactor2: IER3

Evidence Support:


Strong-Evidence Dual luciferase reporter assay//Hybrid-PCR//Luciferase reporter assay//Western blot
Prediction-Evidence cRep//miRanda//PITA//Rep//RNAHybrid//Targetscan
Support Database RepTar//ViRBase//VIRmiRNA//VmiReg

References:


PMID 23403649 Target region 3'UTR
Source RepTar//ViRBase//VIRmiRNA//VmiReg Interactor1 expression None
Tissue or cell line HEK293 cells//HELF cells Interactor2 expression Downregulation
Description The binding site was demonstrated to be functional by dual luciferase reporter assay with a 47% repression of the relative luciferase activity. In an in vitro system of human embryonic kidney 293 (HEK293) cells, the ectopically expressed hcmv-mir-UL148D exhibited a downregulatory effect on IEX-1 expression, and decreased the cell apoptosis induced by transfected IEX-1.

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