Detail Information

Basic Information:


  VIRBase ID:  

VHID00158282

  Virus:  

Human alphaherpesvirus 2 (HSV2)

  Host:  

Homo sapiens

  Confidence Score:  

0.9639

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hsv2-miR-H4-5p:           CDKN2A:      

      *It may take a few minutes to display results.

Interactor Information:


Interactor1 Interactor2
Symbol hsv2-miR-H4-5p CDKN2A
miRBase
Accession/Entrez ID		 MIMAT0010203 1029
Organism Human alphaherpesvirus 2 (HSV2) Homo sapiens
Category miRNA mRNA
Alias - ARF//CDK4I//CDKN2//CMM2//INK4//INK4A//MLM//MTS-1//MTS1//P14//P14ARF//P16//P16-INK4A//P16INK4//P16INK4A//P19//P19ARF//TP16

RNA Editing:


Resource Symbol Editing Position Change Genetic Region
RADAR CDKN2A
chr9:21973986(-) A-I      intronic
chr9:21976678(-) A-I      intronic
chr9:21976376(-) A-I      intronic
chr9:21976543(-) A-I      intronic
chr9:21976632(-) A-I      intronic
chr9:21977544(-) A-I      intronic
chr9:21977564(-) A-I      intronic
chr9:21977461(-) A-I      intronic
chr9:21976296(-) A-I      intronic
Resource Symbol Editing Position Change SeqReg exReg PMID
DARNED CDKN2A
chr9:21977461(-) A-I intron -    22327324

RNA Modification:


Resource Symbol ModificationPosition Type Genomic Context PMID
RMBase CDKN2A
chr9:21968193-21968194(-) m6A 3'UTR       24284625//27773535//22575960
chr9:21968203-21968204(-) m6A 3'UTR       24284625//27773535//22575960
chr9:21968222-21968223(-) m6A 3'UTR//CDS       24284625//27773535//22575960
chr9:21968757-21968758(-) m6A CDS//intron       -
chr9:21969584-21969585(-) m6A 3'UTR//exon//intron       -
chr9:21969604-21969605(-) m6A 3'UTR//exon//intron       -
chr9:21969636-21969637(-) m6A 3'UTR//exon//intron       -
chr9:21970893-21970894(-) m6A CDS//intron       27773536//22575960
chr9:21971010-21971011(-) m6A 3'UTR//CDS//exon       24284625//27773536//22575960
chr9:21971082-21971083(-) m6A 3'UTR//CDS//exon       24284625//27773536//22575960
chr9:21974526-21974527(-) m6A CDS//intron       27773535
chr9:21974886-21974887(-) m6A 5'UTR//intron       24284625//24981863//27773536//22575960
chr9:21974965-21974966(-) m6A 5'UTR//intron       24284625//27773536//22575960
chr9:21989542-21989543(-) m6A exon//intron       -
chr9:21989583-21989584(-) m6A exon//intron       -
chr9:21989608-21989609(-) m6A exon//intron       22608085
chr9:21989672-21989673(-) m6A exon//intron       22575960//22608085
chr9:21989713-21989714(-) m6A exon//intron       24284625//24981863//27773535//22575960//22608085
chr9:21989732-21989733(-) m6A exon//intron       24284625//24981863//27773535//22575960//22608085
chr9:21989756-21989757(-) m6A exon//intron       24284625//24981863//27371828//22575960//22608085
chr9:21989817-21989818(-) m6A exon//intron       24284625//22608085
chr9:21994141-21994142(-) m6A CDS//intron       27773536
chr9:21994331-21994332(-) m6A 5'UTR//intron       24284625//27773536//22575960
chr9:21994911-21994912(-) m6A 5'UTR       24284625
chr9:21995012-21995013(-) m6A 5'UTR       24284625//24981863//22608085

RNA Localization:


Resource Symbol Subcellular Localization Tissue or Cell Line PMID
RNALocate CDKN2A
Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539
Exosome Blood     -
Nucleus HeLa-S3 cells     -
Ribosome HEK-293 cells     22199352

Interaction Network (The top 100 interactions):


Interactor1: hsv2-miR-H4-5p
Interactor2: CDKN2A

Evidence Support:


Strong-Evidence Luciferase reporter assay//qRT-PCR//Western blot
Prediction-Evidence miRanda//RNAHybrid//Targetscan
Support Database ViRBase//VmiReg

References:


PMID 30953292 Target region 3'UTR
Source ViRBase Interactor1 expression Upregulation
Tissue or cell line HeLa cells Interactor2 expression Downregulation
Description Bioinformatics analysis, luciferase report assay, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and Western blotting demonstrated that miRNA-H4-5p could bind to the 3'-untranslated region (UTR) of cyclin-dependent kinase inhibitor 2A (CDKN2A) and cyclin-dependent kinase-like 2 (CDKL2) to negatively regulate their expression.

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