Basic Information:

  VIRBase ID:  

VHID00069829

  Virus:  

Gallid alphaherpesvirus 2

  Host:  

Gallus gallus

  Confidence Score:  

0.9980

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      mdv1-miR-M3-5p:  MIMAT0003923          SMAD2:  NM_204561     

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Interactor Information:

	Interactor1	Interactor2
Symbol	mdv1-miR-M3-5p	SMAD2
miRBase Accession/Entrez ID	MIMAT0003923	395247
Organism	Gallid alphaherpesvirus 2	Gallus gallus
Category	miRNA	mRNA
Alias	-	MADH2//SMAD2Z

Interaction Network (The top 100 interactions):

Interactor1: mdv1-miR-M3-5p
Interactor2: SMAD2

Evidence Support:

Strong-Evidence	Dual luciferase reporter assay//IHC//Luciferase reporter assay//qRT-PCR//RT-PCR//Western blot
Prediction-Evidence	Targetscan
Support Database	ViRBase//VmiReg

References:

[1]PMID	20962090	Target region	3'UTR
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	DF-1 cells//HK293T cells	Interactor2 expression	Downregulation
Description	MDV1-miR-M3 suppressed cisplatin-induced apoptosis by directly downregulating expression at the protein but not the mRNA level of Smad2, a critical component in the transforming growth factor beta signal pathway.

[2]PMID	21277611	Target region	3'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	DF-1 cells//HK293T cells	Interactor2 expression	Downregulation
Description	Indeed, SMAD2 was confirmed as a target that is directly repressed miR-M3, and rescue of SMAD2 expression using a construct lacking the target sites also antagonized the anti-apoptotic effects of the miRNA.