From our rigorous analysis pipeline, which covers three different well-established algorithms (IntaRNA, miRanda, and psRNATarget) to predict RNA-RNA interactions, we have identified 122 and 106 host antiviral miRNAs against SARS-CoV (R) and SARS-CoV-2 (R), respectively (Figures 2A,B, Supplementary File 2).
Among the 2654 human mature miRNAs, 444 miRNAs were identified with direct binding site on different positions along with the coronavirus 2 reference genome (Table S1).
Additionally, if targets are selected by both tools in at least two strains of SARS-CoV-2, we only considered them as potential targets of miRNA on SARS-CoV-2 (Table S2). In Table 1, we present the list of miRNAs meeting all these criteria.
We identified 1,018 miRNAs to target SARS-CoV-2 genes, among which 98 miRNAs are predicted to target SARS-CoV-2 genes uniquely when compared with other viruses studied here (Supplementary Table S6).
