Basic Information:

  VIRBase ID:  

HHID00109699

  Virus:  

Transmissible gastroenteritis virus (Shaanxi strain,TGEV)

  Host:  

Sus scrofa

  Confidence Score:  

0.9630

  Interaction Type:  

Host-Host interaction

  Predicted Binding:

 

      ssc-miR-4331-5p:  MIMAT0037379          RB1:  NM_001285967     

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Interactor Information:

	Interactor1	Interactor2
Symbol	ssc-miR-4331-5p	RB1
miRBase Accession/Entrez ID	MIMAT0037379	100151828
Organism	Sus scrofa	Sus scrofa
Category	miRNA	mRNA
Alias	-	-

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	ssc-miR-4331-5p	Exosome Porcine milk     24499489

Interaction Network (The top 100 interactions):

Interactor1: ssc-miR-4331-5p
Interactor2: RB1

Evidence Support:

Strong-Evidence	Luciferase reporter assay//RT-PCR//Western blot
Prediction-Evidence	miRanda//Targetscan
Support Database	ViRBase

References:

PMID	29217619	Target region	None
Source	ViRBase	Interactor1 expression	Upregulation
Tissue or cell line	PK-15 cells	Interactor2 expression	Downregulation
Description	MiR-4331 directly binds to 3'UTR of RB1 mRNA. To determine whether miR-4331 inhibits RB1 expression, either miR-4331 mimics or miRNA inhibitors control were transfected into PK-15 cells. RB1 protein level was tested using Western blot, showing that expression of RB1 was suppressed by miR-4331 mimics and improved by miR-4331 inhibitors.