Basic Information:

  VIRBase ID:  

HHID00105102

  Virus:  

SARS-CoV-2

  Host:  

Homo sapiens

  Confidence Score:  

0.9644

  Interaction Type:  

Host-Host interaction

  Predicted Binding:

 

      hsa-miR-98-5p:  MIMAT0000096          TMPRSS2:  NM_001135099     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	hsa-miR-98-5p	TMPRSS2
miRBase Accession/Entrez ID	MIMAT0000096	7113
Organism	Homo sapiens	Homo sapiens
Category	miRNA	mRNA
Alias	-	PRSS10

ncRNA SNP information:

Resource	Symbol	SNP ID	SNP Position	Ref/Alt
miRNASNP-v3	hsa-miR-98-5p	rs1357591935 chrX:53556304    T/C rs1556934729 chrX:53556307    A/G rs1556934737 chrX:53556311    T/G rs1556934741 chrX:53556316    C/A

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	TMPRSS2	chr21:42853862(-) A-I      intronic chr21:42853848(-) A-I      intronic chr21:42853888(-) A-I      intronic chr21:42853850(-) A-I      intronic chr21:42853879(-) A-I      intronic chr21:42857482(-) A-I      intronic chr21:42857493(-) A-I      intronic chr21:42857494(-) A-I      intronic chr21:42857532(-) A-I      intronic chr21:42861840(-) A-I      intronic chr21:42863176(-) A-I      intronic chr21:42863181(-) A-I      intronic chr21:42864020(-) A-I      intronic chr21:42857454(-) A-I      intronic chr21:42852821(-) A-I      intronic chr21:42852811(-) A-I      intronic chr21:42868840(-) A-I      intronic chr21:42864037(-) A-I      intronic chr21:42852802(-) A-I      intronic chr21:42852784(-) A-I      intronic chr21:42852783(-) A-I      intronic chr21:42852698(-) A-I      intronic chr21:42868853(-) A-I      intronic chr21:42853773(-) A-I      intronic chr21:42853747(-) A-I      intronic chr21:42852838(-) A-I      intronic chr21:42868879(-) A-I      intronic chr21:42853786(-) A-I      intronic chr21:42853787(-) A-I      intronic chr21:42853866(-) A-I      intronic chr21:42853876(-) A-I      intronic chr21:42853901(-) A-I      intronic chr21:42853902(-) A-I      intronic chr21:42853905(-) A-I      intronic chr21:42853936(-) A-I      intronic chr21:42857436(-) A-I      intronic chr21:42852837(-) A-I      intronic chr21:42852694(-) A-I      intronic chr21:42857467(-) A-I      intronic chr21:42857468(-) A-I      intronic chr21:42857476(-) A-I      intronic chr21:42859902(-) A-I      ncRNA chr21:42859884(-) A-I      ncRNA chr21:42859893(-) A-I      ncRNA chr21:42868835(-) A-I      intronic chr21:42859992(-) A-I      intronic chr21:42855936(-) A-I      intronic chr21:42868944(-) A-I      intronic chr21:42868836(-) A-I      intronic chr21:42852668(-) A-I      intronic chr21:42859993(-) A-I      intronic chr21:42869010(-) A-I      intronic chr21:42863353(-) A-I      intronic chr21:42868900(-) A-I      intronic chr21:42868945(-) A-I      intronic chr21:42852703(-) A-I      intronic chr21:42860827(-) A-I      intronic chr21:42859858(-) A-I      ncRNA chr21:42859831(-) A-I      ncRNA chr21:42859844(-) A-I      ncRNA chr21:42858403(-) A-I      ncRNA

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	TMPRSS2	chr21:42858403(-) A-I intron -    15342557 chr21:42858406(-) A-I intron -    15342557 chr21:42859670(-) A-I intron -    15342557 chr21:42859831(-) A-I intron -    15342557   //15258596 chr21:42859844(-) A-I intron -    15342557   //15258596 chr21:42859858(-) A-I intron -    15342557   //15258596 chr21:42859884(-) A-I intron -    15342557   //15258596 chr21:42859893(-) A-I intron -    15342557   //15258596 chr21:42859902(-) A-I intron -    15342557   //15258596 chr21:42868835(-) A-I intron -    15342557 chr21:42868836(-) A-I intron -    15342557 chr21:42868864(-) A-I intron -    15342557 chr21:42868900(-) A-I intron -    15342557 chr21:42868905(-) A-I intron -    15342557 chr21:42868944(-) A-I intron -    15342557 chr21:42868945(-) A-I intron -    15342557 chr21:42868950(-) A-I intron -    15342557 chr21:42869010(-) A-I intron -    15342557 chr21:42869014(-) A-I intron -    15342557 chr21:42869073(-) A-I intron -    15342557 chr21:42853823(-) A-I intron -    21960545 chr21:42853826(-) A-I intron -    21960545 chr21:42853866(-) A-I intron -    21960545 chr21:42855839(-) A-I intron -    21960545 chr21:42855936(-) A-I intron -    21960545 chr21:42855984(-) A-I intron -    21960545 chr21:42857419(-) A-I intron -    21960545 chr21:42857454(-) A-I intron -    21960545 chr21:42857468(-) A-I intron -    21960545 chr21:42857493(-) A-I intron -    21960545

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	TMPRSS2	chr21:42836773-42836774(-) m6A 3'UTR       25456834

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	hsa-miR-98-5p	Cytoplasm Breast adenocarcinoma cells (MCF-7)|Cervical adenocarcinoma cells (HeLa)     24918059 Exosome Human cervical cancer cell line (HeLa)     25760330 Exosome Primary dendritic cells|T cell line (J77)     21505438 Exosome Breast milk     22211110 Exosome Brain tissue     23382797 Exosome Plasma     23663360 Exosome Human mast cell line (HMC-1)     24009880 Exosome B lymphoblastoid cells|Endothelial cells|Epithelial cells|Mast cells|Tongue tissue     - Microvesicle Plasma     23077538 Microvesicle Seminal plasma     23539611 Microvesicle Colon cancer cell line (LIM1863)     25330373 Microvesicle Colon cancer cell line (LIM1863)|Fibroblasts|Mesenchymal stem cells|Urine     - Mitochondrion Cell line (HEK-293|HeLa)     22984580 Nucleus Breast adenocarcinoma cells (MCF-7)|Cervical adenocarcinoma cells (HeLa)     24918059
	TMPRSS2	Exosome Blood     - Nucleus HCC cell line (HepG2)     - Ribosome Colon cancer cells     24393600

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID	Function
ncDR	hsa-miR-98-5p	Thapsia villosa compd 5477211 Drug sensitive Rogiolol 402519 Drug sensitive N-Hydroxy-N-methylpropanethioamide 371713 Drug sensitive 2-(naphthalen-2-yl)-1-(3,4,5-trimethoxyphenyl)-1h-imidazole - Drug sensitive aquatris(1,3-diphenylpropane-1,3-dionato)neodymium(iii) - Drug sensitive ellipticine, 6-(3-aminopropyl)-, dihydrochloride - Drug sensitive

Resource	Symbol	Drug Name	PubChem ID	Function	Link
RNAInter	hsa-miR-98-5p	10-Hydroxycamptothecin 97226 Drug interaction    RC00001036 Curcumin 969516 Drug interaction    RC00001040 Formaldehyde 712 Drug interaction    RC00001027 Gemcitabine 60750 Drug interaction    RC00001034 Estradiol 5757 Drug interaction    RC00001031 Calcitriol 5280453 Drug interaction    RC00001041 5-Fluorouracil 3385 Drug interaction    RC00001030 Doxorubicin 31703 Drug interaction    RC00001033 cis-Diaminedichloroplatinum 2767 Drug interaction    RC00001029 Celecoxib 2662 Drug interaction    RC00001028 Lenalidomide 216326 Drug interaction    RC00001039 Arsenic trioxide 14888 Drug interaction    RC00001032 Docetaxel 148124 Drug interaction    RC00001038 Ginsenoside Rh2 119307 Drug interaction    RC00001037 Hydroxamic acid HDACi LAQ824 - Drug interaction    RC00001043 1,2,6-Tri-O-galloyl-beta-D-glucopyranose - Drug interaction    RC00001042 Trastuzumab - Drug interaction    RC00001045 LPS - Drug interaction    RC00001044

Resource	Symbol	Drug Name	PubChem ID	PMID
NoncoRNA	hsa-miR-98-5p	Cisplatin 5702198       27270317

Interaction Network (The top 100 interactions):

Interactor1: hsa-miR-98-5p
Interactor2: TMPRSS2

Evidence Support:

Strong-Evidence	Luciferase reporter assay//RT-qPCR//Western blot
Prediction-Evidence	microT//miRanda//miRMap//miRWalk//PicTar//PITA//Targetscan
Support Database	ViRBase

References:

[1]PMID	33428154	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	None	Interactor2 expression	None
Description	To explore the regulatory relationship between the 5 key genes, 3 coronavirus-related genes (ACE2, TMPRSS2, BSG), and their target miRNA, we used 6 miRNA target prediction databases to predict the target miRNA of these genes.

[2]PMID	33143053	Target region	3'UTR
Source	ViRBase	Interactor1 expression	Downregulation
Tissue or cell line	Adult human lung microvascular endothelial cells	Interactor2 expression	Upregulation
Description	The proposed relationship was substantiated by an actual validation of seed complementarity, confirming the interaction between miR-98-5p and TMPRSS2 3'UTR both in HMVEC-L (Figure 2A) and HUVEC (Figure 2B) through a luciferase assay.

[3]PMID	32672403	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	None	Interactor2 expression	None
Description	Hence, the potential role of these gender-associated miRNAs in immunity regulation and in modulation of viral receptors and co-receptors should be considered as a crucial factor in the observed different pathogenicity and lethality of COVID-19 in men and women (see Table 1).

[4]PMID	32814446	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	None	Interactor2 expression	None
Description	Considering the significance of ACE2 and TMPRSS2 in SARS-CoV-2 infection, we also performed a TargetScan (http://www.targetscan.org/vert_72/) search for the miRNAs that could target these two receptors, and the analysis revealed a list of miRNAs such as, hsa-miRNA 200b-3p, hsa-miRNA 200c-3p and miRNA 429 for ACE2, and hsa-let 7c-5p, hsa-miRNA 98-5p, hsa-let 7f-5p, hsa-let 7a-5p, hsa-let 7g-5p, hsa-let 7b-5p, hsa-miRNA 4458, hsa-let 7e-5p, hsa-let 7i-5p, hsa-let 7d-5p, and hsa-miRNA 4500 for TMPRSS2.