Here, we systematically characterize the determinants of the interaction between Lin28 and pre-let-7g by investigating the effect of protein and RNA mutations on in vitro binding. We determine that Lin28 binds with high affinity to the extended loop of pre-let-7g and that its C-terminal domain contributes predominantly to the affinity of this interaction.
Table2 summarizes the list of cellular microRNAs with their validated host cellular protein targets and their corresponding cellular functions.Table 2 List of published cellular microRNAs and their target HIV-1 and cellular gene products.