Basic Information:

  VIRBase ID:  

HHID00044910

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9630

  Interaction Type:  

Host-Host interaction

  Predicted Binding:

 

      hsa-miR-31-5p:  MIMAT0000089          HIF1AN:  NM_017902     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hsa-miR-31-5p	HIF1AN
miRBase Accession/Entrez ID	MIMAT0000089	55662
Organism	Homo sapiens	Homo sapiens
Category	miRNA	mRNA
Alias	-	FIH1

ncRNA SNP information:

Resource	Symbol	SNP ID	SNP Position	Ref/Alt
miRNASNP-v3	hsa-miR-31-5p	rs368583676 chr9:21512165    C/T rs1475828657 chr9:21512176    C/A

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	HIF1AN	chr10:102300880(+) A-I      intronic chr10:102301281(+) A-I      intronic chr10:102302188(+) A-I      intronic chr10:102303522(+) A-I      intronic chr10:102303540(+) A-I      intronic chr10:102303046(+) A-I      intronic chr10:102300765(+) A-I      intronic chr10:102302319(+) A-I      intronic chr10:102298780(+) A-I      intronic chr10:102303047(+) A-I      intronic chr10:102298314(+) A-I      intronic chr10:102303048(+) A-I      intronic chr10:102303375(+) A-I      intronic chr10:102298686(+) A-I      intronic chr10:102298732(+) A-I      intronic chr10:102298762(+) A-I      intronic chr10:102298763(+) A-I      intronic chr10:102300716(+) A-I      intronic chr10:102303386(+) A-I      intronic chr10:102300717(+) A-I      intronic chr10:102300789(+) A-I      intronic chr10:102300794(+) A-I      intronic chr10:102298319(+) A-I      intronic chr10:102304079(+) A-I      intronic chr10:102300739(+) A-I      intronic chr10:102300901(+) A-I      intronic chr10:102303536(+) A-I      intronic chr10:102302374(+) A-I      intronic chr10:102301014(+) A-I      intronic chr10:102298467(+) A-I      intronic chr10:102300109(+) A-I      intronic chr10:102301076(+) A-I      intronic chr10:102301279(+) A-I      intronic chr10:102300046(+) A-I      intronic chr10:102304078(+) A-I      intronic chr10:102301027(+) A-I      intronic chr10:102301375(+) A-I      intronic chr10:102301097(+) A-I      intronic chr10:102300086(+) A-I      intronic chr10:102298789(+) A-I      intronic chr10:102302323(+) A-I      intronic chr10:102302373(+) A-I      intronic chr10:102301087(+) A-I      intronic chr10:102301414(+) A-I      intronic chr10:102298457(+) A-I      intronic chr10:102298792(+) A-I      intronic chr10:102298489(+) A-I      intronic chr10:102298498(+) A-I      intronic chr10:102303232(+) A-I      intronic chr10:102301338(+) A-I      intronic chr10:102298963(+) A-I      intronic chr10:102301289(+) A-I      intronic chr10:102301005(+) A-I      intronic chr10:102298326(+) A-I      intronic chr10:102298358(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	HIF1AN	chr10:102300086(+) A-G intron -    22484847 chr10:102300109(+) A-G intron -    22484847 chr10:102301014(+) A-G intron -    22484847 chr10:102301375(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	HIF1AN	chr10:102295975-102295976(+) m6A exon//intron       - chr10:102296382-102296383(+) m6A 3'UTR//CDS//exon       24981863//26404942 chr10:102300443-102300444(+) m6A 3'UTR//CDS//exon       24981863 chr10:102306963-102306964(+) m6A CDS       24209618//26404942 chr10:102306996-102306997(+) m6A CDS       24209618//26404942 chr10:102307865-102307866(+) m6A CDS       24284625//24209618//26404942//22608085 chr10:102307960-102307961(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22608085 chr10:102307967-102307968(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22608085 chr10:102308003-102308004(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22608085 chr10:102308008-102308009(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22608085 chr10:102308082-102308083(+) m6A 3'UTR       24284625//24209618//25456834//24981863//22608085 chr10:102308470-102308471(+) m6A 3'UTR       24981863//27773535//22575960 chr10:102308588-102308589(+) m6A 3'UTR       24981863//22608085 chr10:102308596-102308597(+) m6A 3'UTR       24981863//22608085 chr10:102308604-102308605(+) m6A 3'UTR       24981863//22608085 chr10:102308636-102308637(+) m6A 3'UTR       24981863 chr10:102309108-102309109(+) Y 3'UTR       26075521 chr10:102310341-102310342(+) m6A 3'UTR       - chr10:102310419-102310420(+) m6A 3'UTR       - chr10:102311610-102311611(+) m6A 3'UTR       - chr10:102311622-102311623(+) m6A 3'UTR       - chr10:102311641-102311642(+) m6A 3'UTR       - chr10:102312331-102312332(+) Y 3'UTR       26075521 chr10:102316120-102316121(+) m6A 3'UTR       24981863 chr10:102316185-102316186(+) m6A 3'UTR       24981863 chr10:102316308-102316309(+) m6A 3'UTR       24981863 chr10:102316840-102316841(+) m6A 3'UTR       24981863 chr10:102316874-102316875(+) m6A 3'UTR       24981863 chr10:102316880-102316881(+) m6A 3'UTR       24981863 chr10:102316886-102316887(+) m6A 3'UTR       24981863 chr10:102316957-102316958(+) m6A 3'UTR       24981863 chr10:102316981-102316982(+) m6A 3'UTR       24981863 chr10:102317002-102317003(+) m6A 3'UTR       24981863 chr10:102317819-102317820(+) m6A 3'UTR       24981863

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	hsa-miR-31-5p	Apoptotic body Human umbilical vein endothelial cells     19996457 Cytoplasm Cell line (HeLa)     21695135 Cytoplasm Breast adenocarcinoma cells (MCF-7)|Cervical adenocarcinoma cells (HeLa)     24918059 Exosome Human cervical cancer cell line (HeLa)     29492481 Exosome Breast milk     22211110 Exosome Brain tissue     23382797 Exosome Plasma     23663360 Exosome Endothelial cells|Tongue tissue     - Extracellular vesicle HEK-293T cells     22849433 Microvesicle Seminal plasma     23539611 Microvesicle Follicular fluid     23666971 Microvesicle Colon cancer cell line (LIM1863)|Fibroblasts|Mesenchymal stem cells|Urine     - Mitochondrion Skeletal muscle myoblasts     21637849 Mitochondrion Cell line (HEK-293|HeLa)     22984580 Nucleolus Breast adenocarcinoma cells (MCF-7)|Cervical adenocarcinoma cells (HeLa)     24918059 Nucleus Breast adenocarcinoma cells (MCF-7)|Cervical adenocarcinoma cells (HeLa)     24918059
	HIF1AN	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells     - Ribosome Colon cancer cells     24393600

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID	Function
ncDR	hsa-miR-31-5p	Midostaurin 9829523 Drug sensitive Dichlorodipropylstannane 93562 Drug resistant Pyrazolo[1,5-b]pyridazine deriv. 40 6539382 Drug sensitive (Z)-4-Bromo-4-iodo-3-phenylbut-3-en-2-one 5467656 Drug resistant Erioflorin acetate 5281443 Drug resistant 10-Hydroxy-1,2,6a,6b,9,9,12a-heptamethyl-2,3,4,5,6,6a,7,8,8a,10,11,12-dodecahydro-1H-picene-4a-carboxylic acid 495604 Drug sensitive 3-(4-Fluorophenyl)-3-(4-hydroxy-2-methylphenyl)-2-benzofuran-1(3H)-one 387973 Drug sensitive 2,5-Bis(3-Amino-2,4,6-trinitrophenyl)-1,3,4-thiadiazole 385374 Drug resistant 7-Chloro-6-(2-fluoroethylamino)quinoline-5,8-dione 379079 Drug resistant 4-(1-Benzofuran-2-yl)-7-methoxychromen-2-one 364364 Drug sensitive Girolline 362388 Drug sensitive 4-Chloro-5-phenoxy-3H-1,2-dithiole-3-thione 16766044 Drug resistant 2,2-Diethyl-6-(hydroxymethyl)-4,6,7,7a-tetrahydro-3aH-[1,3,2]dioxastannolo[4,5-c]pyran-4,7-diol 16683462 Drug resistant Inosine dialdehyde 135400916 Drug resistant Melampodinin 11953936 Drug resistant 3-Bromo-4-(3,3-dimethyl-but-1-ynyl)-2(5H)-furanone 11413833 Drug sensitive 7h-5,6-dithioleno[4,3-d]uracil - Drug resistant benzene, 1,1'-[(1,3-butadiene-1,4-diyl)sulfonyl]bis - Drug resistant benzo[1,2-c:4,5-c']dipyrrole-1,3,5,7(2h,6h)-tetraimine - Drug resistant [bis(aminomethyl)diethylsilane]dichloroplatinum (ii) - Drug resistant 5-hydroxyamino camptothecin - Drug resistant indole-2,3-dione, 5-methyl-, 3-[(o-nitrophenyl)hydrazone] - Drug sensitive antineoplastic-d655481 - Drug sensitive tp4ek - Drug sensitive

Resource	Symbol	Drug Name	PubChem ID	Function	Link
RNAInter	hsa-miR-31-5p	10-Hydroxycamptothecin 97226 Drug interaction    RC00000917 Gemcitabine 60750 Drug interaction    RC00000916 Vincristine 5978 Drug interaction    RC00000910 Sunitinib 5329102 Drug interaction    RC00000925 Vorinostat 5311 Drug interaction    RC00000924 Eloxatine 5310940 Drug interaction    RC00000923 (5Z,13E)-(15S)-6,9alpha-Epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate 5280427 Drug interaction    RC00000922 Decitabine 451668 Drug interaction    RC00000921 Trichostatin A 444732 Drug interaction    RC00000920 Staurosporine 44259 Drug interaction    RC00000915 Etoposide 36462 Drug interaction    RC00000914 Paclitaxel 36314 Drug interaction    RC00000913 5-Fluorouracil 3385 Drug interaction    RC00000909 Enoxacin 3229 Drug interaction    RC00000908 Doxorubicin 31703 Drug interaction    RC00000912 Valproic acid 3121 Drug interaction    RC00000907 cis-Diaminedichloroplatinum 2767 Drug interaction    RC00000906 Verapamil 2520 Drug interaction    RC00000905 Calcium sulfate 24497 Drug interaction    RC00000911 Docetaxel 148124 Drug interaction    RC00000919 Gefitinib 123631 Drug interaction    RC00000918 Succinic acid 1110 Drug interaction    RC00000904 platinum-based doublet chemotherapy - Drug interaction    RC00000926 5-aza-2'-deoxycytidine (5-Aza-CdR) + trichostatin A(TSA) - Drug interaction    RC00000927

Resource	Symbol	Drug Name	PubChem ID	PMID
NoncoRNA	hsa-miR-31-5p	Oxaliplatin 9887053       30400960 Cisplatin 5702198       25356050 5-Fluorouracil 3385       25356050

Interaction Network (The top 100 interactions):

Interactor1: hsa-miR-31-5p
Interactor2: HIF1AN

Evidence Support:

Strong-Evidence	Luciferase reporter assay//qRT-PCR//Western blot
Prediction-Evidence	miRanda//Targetscan
Support Database	ViRBase

References:

PMID	25098679	Target region	3'UTR
Source	ViRBase	Interactor1 expression	Downregulation
Tissue or cell line	C15 cells//C17 cells//C666-1 cells//X666 cells//X1915 cells//X2117 cells//X99186 cells	Interactor2 expression	Downregulation
Description	By luciferase reporter assay, FIH1 and MCM2 were further confirmed to be direct targets of miR-31 in C666-1. The binding of miR-31 to the 3'UTR of these genes markedly inhibited luciferase activity (Figure 5b). As shown in Figure 5, miR-31 highly suppressed the expression of MCM2 and FIH1 in NPC cells.