Basic Information:

  VIRBase ID:  

HHID00019428

  Virus:  

Hepacivirus C (Hepatitis C virus JFH-1,HCV)

  Host:  

Homo sapiens

  Confidence Score:  

0.8589

  Interaction Type:  

Host-Host interaction

  Predicted Binding:

 

      hsa-miR-30b-3p:  MIMAT0004589          IFNB1:  NM_002176     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hsa-miR-30b-3p	IFNB1
miRBase Accession/Entrez ID	MIMAT0004589	3456
Organism	Homo sapiens	Homo sapiens
Category	miRNA	mRNA
Alias	-	IFB//IFF//IFN-beta//IFNB

ncRNA SNP information:

Resource	Symbol	SNP ID	SNP Position	Ref/Alt
miRNASNP-v3	hsa-miR-30b-3p	rs1160794252 chr8:134800541    A/G rs761170520 chr8:134800545    A/C rs776207215 chr8:134800535    G/GT rs1293522812 chr8:134800547    C/T rs1407185230 chr8:134800550    C/T rs1435060162 chr8:134800546    C/T rs766838703 chr8:134800548    T/C

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	IFNB1	chr9:21077198-21077199(-) m6A 3'UTR       27773535 chr9:21077273-21077274(-) m6A 3'UTR       27773535 chr9:21077278-21077279(-) m6A 3'UTR       27773535 chr9:21077308-21077309(-) m6A CDS       27773535 chr9:21077328-21077329(-) m6A CDS       27773535 chr9:21077347-21077348(-) m6A CDS       27773535 chr9:21077375-21077376(-) m6A CDS       27773535 chr9:21077592-21077593(-) m6A CDS       27773535 chr9:21077611-21077612(-) m6A CDS       27773535 chr9:21077695-21077696(-) m6A CDS       27773535 chr9:21077704-21077705(-) m6A CDS       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	hsa-miR-30b-3p	Exosome Breast milk     22211110 Exosome Plasma     23663360 Exosome Human mast cell line (HMC-1)     24009880 Exosome Breast milk|Cell lines|Epididymal epithelial cells|Osteoblast|Plasma|Primary glioblastoma neurosphere cells|Primary keratinocyte|Saliva|Serum|Urine     - Microvesicle Seminal plasma     23539611 Microvesicle Fibroblasts|Mesenchymal stem cells|Urine     -

Interaction Network (The top 100 interactions):

Interactor1: hsa-miR-30b-3p
Interactor2: IFNB1

Evidence Support:

Strong-Evidence	RT-PCR
Weak-Evidence	Microarray
Support Database	VIRmiRNA

References:

PMID	17943132	Target region	None
Source	VIRmiRNA	Interactor1 expression	None
Tissue or cell line	Huh7 cells	Interactor2 expression	None
Description	Transfection of the eight candidate miRNAs individually revealed that miRNAs miR-196, miR-296, miR-351, miR-431 and miR-448 were indeed able to substantially attenuate viral replication, whereas miRNAs miR-1, miR-30 and miR-128 were without effect (Fig. 2a). Transfection of a mixture of the five functional miRNA-mimics yielded a >80% reduction in viral RNA load. As IFNbeta induces miRNAs miR-196, miR-296, miR-351, miR-431 and miR-448 but downregulates miR-122, we decided to imitate this cellular response by transfecting the mix of miRNA-mimics in combination with anti-miR-122.