Basic Information:

  VIRBase ID:  

HHID00019416

  Virus:  

Hepacivirus C (Hepatitis C virus JFH-1,HCV)

  Host:  

Homo sapiens

  Confidence Score:  

0.8589

  Interaction Type:  

Host-Host interaction

  Predicted Binding:

 

      hsa-miR-30e-3p:  MIMAT0000693          IFNB1:  NM_002176     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hsa-miR-30e-3p	IFNB1
miRBase Accession/Entrez ID	MIMAT0000693	3456
Organism	Homo sapiens	Homo sapiens
Category	miRNA	mRNA
Alias	-	IFB//IFF//IFN-beta//IFNB

ncRNA SNP information:

Resource	Symbol	SNP ID	SNP Position	Ref/Alt
miRNASNP-v3	hsa-miR-30e-3p	rs1184629069 chr1:40754434    C/T rs1217267713 chr1:40754424    A/C rs1485494658 chr1:40754429    T/G rs538913221 chr1:40754422    G/A rs756374506 chr1:40754425    T/C rs767610978 chr1:40754421    C/A rs767610978 chr1:40754421    C/T rs976303699 chr1:40754432    A/G rs367890071 chr1:40754418    A/G rs373732123 chr1:40754417    C/T

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	IFNB1	chr9:21077198-21077199(-) m6A 3'UTR       27773535 chr9:21077273-21077274(-) m6A 3'UTR       27773535 chr9:21077278-21077279(-) m6A 3'UTR       27773535 chr9:21077308-21077309(-) m6A CDS       27773535 chr9:21077328-21077329(-) m6A CDS       27773535 chr9:21077347-21077348(-) m6A CDS       27773535 chr9:21077375-21077376(-) m6A CDS       27773535 chr9:21077592-21077593(-) m6A CDS       27773535 chr9:21077611-21077612(-) m6A CDS       27773535 chr9:21077695-21077696(-) m6A CDS       27773535 chr9:21077704-21077705(-) m6A CDS       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	hsa-miR-30e-3p	Exosome Primary dendritic cells|T cell line (J77)     21505438 Exosome Breast milk     22211110 Exosome Brain tissue     23382797 Exosome Plasma     23663360 Exosome Human mast cell line (HMC-1)     24009880 Exosome B lymphoblastoid cells|Chronic lymphocytic leukemia cells|Endothelial cells|Lymphoma tissue|Mast cells     - Microvesicle Seminal plasma     23539611 Microvesicle Follicular fluid     23666971 Microvesicle Serum     24797360 Microvesicle Colon cancer cell line (LIM1863)|Fibroblasts|Mesenchymal stem cells|Urine     - Mitochondrion Skeletal muscle myoblasts     21637849 Mitochondrion Cell line (HEK-293|HeLa)     22984580

Interaction Network (The top 100 interactions):

Interactor1: hsa-miR-30e-3p
Interactor2: IFNB1

Evidence Support:

Strong-Evidence	RT-PCR
Weak-Evidence	Microarray
Support Database	VIRmiRNA

References:

PMID	17943132	Target region	None
Source	VIRmiRNA	Interactor1 expression	None
Tissue or cell line	Huh7 cells	Interactor2 expression	None
Description	Transfection of the eight candidate miRNAs individually revealed that miRNAs miR-196, miR-296, miR-351, miR-431 and miR-448 were indeed able to substantially attenuate viral replication, whereas miRNAs miR-1, miR-30 and miR-128 were without effect (Fig. 2a). Transfection of a mixture of the five functional miRNA-mimics yielded a >80% reduction in viral RNA load. As IFNbeta induces miRNAs miR-196, miR-296, miR-351, miR-431 and miR-448 but downregulates miR-122, we decided to imitate this cellular response by transfecting the mix of miRNA-mimics in combination with anti-miR-122.