Basic Information:

  VIRBase ID:  

HHID00016690

  Virus:  

Zaire ebolavirus (ZEBOV)

  Host:  

Homo sapiens

  Confidence Score:  

0.5908

  Interaction Type:  

Host-Host interaction

  Predicted Binding:

 

      hsa-miR-4521:  MIMAT0019058          CLMP:  NM_024769     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	hsa-miR-4521	CLMP
miRBase Accession/Entrez ID	MIMAT0019058	79827
Organism	Homo sapiens	Homo sapiens
Category	miRNA	mRNA
Alias	-	ACAM//ASAM//CSBM//CSBS

ncRNA SNP information:

Resource	Symbol	SNP ID	SNP Position	Ref/Alt
miRNASNP-v3	hsa-miR-4521	rs1011991709 chr17:8186962    G/A rs1011991709 chr17:8186962    G/C rs1041768648 chr17:8186965    C/T rs1056190393 chr17:8186959    C/A rs1231036635 chr17:8186958    T/A rs1231036635 chr17:8186958    T/G rs1316923680 chr17:8186964    G/A rs1316923680 chr17:8186964    G/C rs1316923680 chr17:8186964    G/T rs1365122718 chr17:8186957    G/C rs559116584 chr17:8186963    T/C rs559116584 chr17:8186963    T/G rs561824123 chr17:8186969    G/A rs768576971 chr17:8186966    T/A rs768576971 chr17:8186966    T/C rs776348878 chr17:8186967    C/A rs893173803 chr17:8186960    C/G rs893173803 chr17:8186960    C/T rs999343007 chr17:8186968    A/G rs1310598293 chr17:8186951    A/G rs928871091 chr17:8186953    G/A rs928871091 chr17:8186953    G/C rs937623219 chr17:8186954    G/A rs953552065 chr17:8186949    C/A rs953552065 chr17:8186949    C/G rs953552065 chr17:8186949    C/T rs962385560 chr17:8186955    A/G

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	CLMP	chr11:122997954(-) A-I      intronic chr11:122998008(-) A-I      intronic chr11:122943074(-) A-I      3'UTR chr11:122943033(-) A-I      3'UTR chr11:122948623(-) A-I      intronic chr11:122948207(-) A-I      intronic chr11:122948212(-) A-I      intronic chr11:122998003(-) A-I      intronic chr11:122948610(-) A-I      intronic chr11:122948265(-) A-I      intronic chr11:122948214(-) A-I      intronic chr11:122948262(-) A-I      intronic chr11:122948221(-) A-I      intronic chr11:122997999(-) A-I      intronic chr11:122948251(-) A-I      intronic chr11:122946715(-) A-I      intronic chr11:122946724(-) A-I      intronic chr11:122997986(-) A-I      intronic chr11:122952411(-) A-I      intronic chr11:122997967(-) A-I      intronic chr11:122997958(-) A-I      intronic chr11:122948682(-) A-I      intronic chr11:122948675(-) A-I      intronic chr11:122948632(-) A-I      intronic chr11:123043407(-) A-I      intronic chr11:122948738(-) A-I      intronic chr11:122951147(-) A-I      intronic chr11:122953125(-) A-I      intronic chr11:123043395(-) A-I      intronic chr11:122975131(-) A-I      intronic chr11:122975134(-) A-I      intronic chr11:122943119(-) A-I      3'UTR chr11:122948728(-) A-I      intronic chr11:122948227(-) A-I      intronic chr11:122975155(-) A-I      intronic chr11:122975171(-) A-I      intronic chr11:122975190(-) A-I      intronic chr11:122983148(-) A-I      intronic chr11:122983151(-) A-I      intronic chr11:122983154(-) A-I      intronic chr11:122948204(-) A-I      intronic chr11:122997892(-) A-I      intronic chr11:122997913(-) A-I      intronic chr11:122997922(-) A-I      intronic chr11:123043374(-) A-I      intronic chr11:123043361(-) A-I      intronic chr11:123043360(-) A-I      intronic chr11:123043358(-) A-I      intronic chr11:123043354(-) A-I      intronic chr11:123043330(-) A-I      intronic chr11:123043328(-) A-I      intronic chr11:123043318(-) A-I      intronic chr11:122998012(-) A-I      intronic chr11:122997941(-) A-I      intronic chr11:122998009(-) A-I      intronic chr11:122943100(-) A-I      3'UTR chr11:122992117(-) A-I      intronic chr11:122948307(-) A-I      intronic chr11:122992095(-) A-I      intronic chr11:123044615(-) A-I      intronic chr11:122990872(-) A-I      intronic chr11:122990611(-) A-I      intronic chr11:122990603(-) A-I      intronic chr11:122990028(-) A-I      intronic chr11:122990015(-) A-I      intronic chr11:122989973(-) A-I      intronic chr11:122989970(-) A-I      intronic chr11:122989969(-) A-I      intronic chr11:122989930(-) A-I      intronic chr11:122989929(-) A-I      intronic chr11:122989920(-) A-I      intronic chr11:122989903(-) A-I      intronic chr11:122989854(-) A-I      intronic chr11:122946206(-) A-I      intronic chr11:122988923(-) A-I      intronic chr11:122988906(-) A-I      intronic chr11:122988905(-) A-I      intronic chr11:122988329(-) A-I      intronic chr11:122948241(-) A-I      intronic chr11:122988314(-) A-I      intronic chr11:122987193(-) A-I      intronic chr11:122983197(-) A-I      intronic chr11:122983183(-) A-I      intronic chr11:122983161(-) A-I      intronic chr11:122981067(-) A-I      intronic chr11:122973165(-) A-I      intronic chr11:122948234(-) A-I      intronic chr11:122957582(-) A-I      intronic chr11:122953533(-) A-I      intronic chr11:122953484(-) A-I      intronic chr11:122946290(-) A-I      intronic chr11:122953480(-) A-I      intronic chr11:122953447(-) A-I      intronic chr11:122947831(-) A-I      intronic chr11:122946298(-) A-I      intronic chr11:122948200(-) A-I      intronic chr11:122953439(-) A-I      intronic chr11:122953430(-) A-I      intronic chr11:122953205(-) A-I      intronic chr11:122953177(-) A-I      intronic chr11:122946300(-) A-I      intronic chr11:122953155(-) A-I      intronic chr11:122948108(-) A-I      intronic chr11:122945875(-) A-I      intronic chr11:123044642(-) A-I      intronic chr11:123044715(-) A-I      intronic chr11:123044722(-) A-I      intronic chr11:123044739(-) A-I      intronic chr11:123054041(-) A-I      intronic chr11:123060543(-) A-I      intronic chr11:122952387(-) A-I      intronic chr11:122947920(-) A-I      intronic chr11:122948698(-) A-I      intronic chr11:122947934(-) A-I      intronic chr11:123043373(-) A-I      intronic chr11:122995729(-) A-I      intronic chr11:123007489(-) A-I      intronic chr11:122997529(-) A-I      intronic chr11:122948692(-) A-I      intronic chr11:122951648(-) A-I      intronic chr11:122949262(-) A-I      intronic chr11:122952448(-) A-I      intronic chr11:122992697(-) A-I      intronic chr11:122949625(-) A-I      intronic chr11:122972647(-) A-I      intronic chr11:123043359(-) A-I      intronic chr11:122948178(-) A-I      intronic chr11:122947871(-) A-I      intronic chr11:122953557(-) A-I      intronic chr11:123043282(-) A-I      intronic chr11:122947845(-) A-I      intronic chr11:122991760(-) A-I      intronic chr11:122951064(-) A-I      intronic chr11:122948649(-) A-I      intronic chr11:123043287(-) A-I      intronic chr11:123043344(-) A-I      intronic chr11:122952458(-) A-I      intronic chr11:123043349(-) A-I      intronic chr11:122967616(-) A-I      intronic chr11:123043350(-) A-I      intronic chr11:122953154(-) A-I      intronic chr11:122990884(-) A-I      intronic chr11:123042262(-) A-I      intronic chr11:122953149(-) A-I      intronic chr11:122947900(-) A-I      intronic chr11:122987791(-) A-I      intronic chr11:123044605(-) A-I      intronic chr11:122952394(-) A-I      intronic chr11:122953141(-) A-I      intronic chr11:123043431(-) A-I      intronic chr11:122996507(-) A-I      intronic chr11:122990973(-) A-I      intronic chr11:122948671(-) A-I      intronic chr11:123042290(-) A-I      intronic chr11:122951839(-) A-I      intronic chr11:123043440(-) A-I      intronic chr11:123044572(-) A-I      intronic chr11:122999819(-) A-I      intronic chr11:122949627(-) A-I      intronic chr11:122951822(-) A-I      intronic chr11:122945828(-) A-I      intronic chr11:122948563(-) A-I      intronic chr11:123044598(-) A-I      intronic chr11:122952413(-) A-I      intronic chr11:122948702(-) A-I      intronic chr11:122952353(-) A-I      intronic chr11:122952343(-) A-I      intronic chr11:123044607(-) A-I      intronic chr11:122952336(-) A-I      intronic chr11:122952316(-) A-I      intronic chr11:122995010(-) A-I      intronic chr11:122946316(-) A-I      intronic chr11:122951742(-) A-I      intronic chr11:122951302(-) A-I      intronic chr11:122946689(-) A-I      intronic chr11:123043281(-) A-I      intronic chr11:123043079(-) A-I      intronic chr11:123043020(-) A-I      intronic chr11:122963058(-) A-I      intronic chr11:122967108(-) A-I      intronic chr11:123042987(-) A-I      intronic chr11:122989021(-) A-I      intronic chr11:122949252(-) A-I      intronic chr11:122951301(-) A-I      intronic chr11:123042984(-) A-I      intronic chr11:123042980(-) A-I      intronic chr11:123041367(-) A-I      intronic chr11:122951277(-) A-I      intronic chr11:122951266(-) A-I      intronic chr11:123041362(-) A-I      intronic chr11:122946818(-) A-I      intronic chr11:123041327(-) A-I      intronic chr11:123041322(-) A-I      intronic chr11:122951223(-) A-I      intronic chr11:123041280(-) A-I      intronic chr11:122951184(-) A-I      intronic chr11:123032350(-) A-I      intronic chr11:123020792(-) A-I      intronic chr11:123016447(-) A-I      intronic chr11:123016238(-) A-I      intronic chr11:122951141(-) A-I      intronic chr11:123015328(-) A-I      intronic chr11:122947772(-) A-I      intronic chr11:123013473(-) A-I      intronic chr11:122951138(-) A-I      intronic chr11:123012966(-) A-I      intronic chr11:123012958(-) A-I      intronic chr11:123012910(-) A-I      intronic chr11:122950368(-) A-I      intronic chr11:122947783(-) A-I      intronic chr11:122950359(-) A-I      intronic chr11:123012905(-) A-I      intronic chr11:123012889(-) A-I      intronic chr11:123010341(-) A-I      intronic chr11:122950348(-) A-I      intronic chr11:123010281(-) A-I      intronic chr11:122949699(-) A-I      intronic chr11:122949619(-) A-I      intronic chr11:122973551(-) A-I      intronic chr11:123009562(-) A-I      intronic chr11:122949557(-) A-I      intronic chr11:122949555(-) A-I      intronic chr11:123009467(-) A-I      intronic chr11:123009447(-) A-I      intronic chr11:122949542(-) A-I      intronic chr11:123008042(-) A-I      intronic chr11:123008036(-) A-I      intronic chr11:123008026(-) A-I      intronic chr11:123007973(-) A-I      intronic chr11:123007909(-) A-I      intronic chr11:122953386(-) A-I      intronic chr11:122949527(-) A-I      intronic chr11:123007884(-) A-I      intronic chr11:122949491(-) A-I      intronic chr11:122949485(-) A-I      intronic chr11:122949464(-) A-I      intronic chr11:122947818(-) A-I      intronic chr11:123043099(-) A-I      intronic chr11:123007548(-) A-I      intronic chr11:123005820(-) A-I      intronic chr11:122949293(-) A-I      intronic chr11:122949257(-) A-I      intronic chr11:123005819(-) A-I      intronic chr11:123005805(-) A-I      intronic chr11:123005802(-) A-I      intronic chr11:123001653(-) A-I      intronic chr11:122949240(-) A-I      intronic chr11:122951103(-) A-I      intronic chr11:123000031(-) A-I      intronic chr11:122999991(-) A-I      intronic chr11:123044533(-) A-I      intronic chr11:122996508(-) A-I      intronic chr11:122996493(-) A-I      intronic chr11:122996479(-) A-I      intronic chr11:122949227(-) A-I      intronic chr11:122994185(-) A-I      intronic chr11:122994184(-) A-I      intronic chr11:122952470(-) A-I      intronic chr11:122994174(-) A-I      intronic chr11:122992287(-) A-I      intronic chr11:122992285(-) A-I      intronic chr11:122992262(-) A-I      intronic chr11:122992655(-) A-I      intronic chr11:122992209(-) A-I      intronic chr11:122992184(-) A-I      intronic chr11:122992144(-) A-I      intronic chr11:122947827(-) A-I      intronic chr11:122949325(-) A-I      intronic chr11:122943093(-) A-I      3'UTR chr11:122943082(-) A-I      3'UTR chr11:122943064(-) A-I      3'UTR chr11:122943085(-) A-I      3'UTR chr11:122943039(-) A-I      3'UTR chr11:122943050(-) A-I      3'UTR chr11:122943052(-) A-I      3'UTR chr11:122943069(-) A-I      3'UTR chr11:122943114(-) A-I      3'UTR chr11:122943109(-) A-I      3'UTR chr11:122952442(-) A-I      intronic chr11:122990961(-) A-I      intronic chr11:122971992(-) A-I      intronic chr11:122950316(-) A-I      intronic chr11:122999787(-) A-I      intronic chr11:122953235(-) A-I      intronic chr11:123032256(-) A-I      intronic chr11:123012989(-) A-I      intronic chr11:123042931(-) A-I      intronic chr11:122953594(-) A-I      intronic chr11:123044610(-) A-I      intronic chr11:123031429(-) A-I      intronic chr11:123002426(-) A-I      intronic chr11:122999824(-) A-I      intronic chr11:122995195(-) A-I      intronic chr11:122946769(-) A-I      intronic chr11:122951253(-) A-I      intronic chr11:122945756(-) A-I      intronic chr11:122946821(-) A-I      intronic chr11:122953587(-) A-I      intronic chr11:122946780(-) A-I      intronic chr11:122953528(-) A-I      intronic chr11:123032276(-) A-I      intronic chr11:123035097(-) A-I      intronic chr11:122949231(-) A-I      intronic chr11:122995782(-) A-I      intronic chr11:122951730(-) A-I      intronic chr11:122951823(-) A-I      intronic chr11:122951609(-) A-I      intronic chr11:122987070(-) A-I      intronic chr11:123010300(-) A-I      intronic chr11:122991728(-) A-I      intronic chr11:123015329(-) A-I      intronic chr11:122953113(-) A-I      intronic chr11:122978031(-) A-I      intronic chr11:123042948(-) A-I      intronic chr11:122950235(-) A-I      intronic chr11:122947924(-) A-I      intronic chr11:122946840(-) A-I      intronic chr11:123042398(-) A-I      intronic chr11:122951859(-) A-I      intronic chr11:122990937(-) A-I      intronic chr11:122951779(-) A-I      intronic chr11:122949237(-) A-I      intronic chr11:123009633(-) A-I      intronic chr11:122948809(-) A-I      intronic chr11:122995779(-) A-I      intronic chr11:122946718(-) A-I      intronic chr11:122972662(-) A-I      intronic chr11:123009566(-) A-I      intronic chr11:123010329(-) A-I      intronic chr11:122947826(-) A-I      intronic chr11:123007414(-) A-I      intronic chr11:123042958(-) A-I      intronic chr11:122990484(-) A-I      intronic chr11:123009389(-) A-I      intronic chr11:122995707(-) A-I      intronic chr11:123012801(-) A-I      intronic chr11:122951785(-) A-I      intronic chr11:122994262(-) A-I      intronic chr11:122985861(-) A-I      intronic chr11:122947880(-) A-I      intronic chr11:123024620(-) A-I      intronic chr11:122949190(-) A-I      intronic chr11:123024667(-) A-I      intronic chr11:122948652(-) A-I      intronic chr11:122990926(-) A-I      intronic chr11:122947774(-) A-I      intronic chr11:122972674(-) A-I      intronic chr11:122953516(-) A-I      intronic chr11:122989985(-) A-I      intronic chr11:122990911(-) A-I      intronic chr11:123043389(-) A-I      intronic chr11:122946624(-) A-I      intronic chr11:122948801(-) A-I      intronic chr11:122946638(-) A-I      intronic chr11:122951792(-) A-I      intronic chr11:122951732(-) A-I      intronic chr11:122953122(-) A-I      intronic chr11:122951719(-) A-I      intronic chr11:122946805(-) A-I      intronic chr11:122991670(-) A-I      intronic chr11:122951761(-) A-I      intronic chr11:122974959(-) A-I      intronic chr11:122948781(-) A-I      intronic chr11:123009659(-) A-I      intronic chr11:122956892(-) A-I      intronic chr11:122945894(-) A-I      intronic chr11:123014720(-) A-I      intronic chr11:122995785(-) A-I      intronic chr11:123042289(-) A-I      intronic chr11:123044360(-) A-I      intronic chr11:122946637(-) A-I      intronic chr11:122999788(-) A-I      intronic chr11:122958171(-) A-I      intronic chr11:122999863(-) A-I      intronic chr11:122951855(-) A-I      intronic chr11:122992648(-) A-I      intronic chr11:122972745(-) A-I      intronic chr11:122990674(-) A-I      intronic chr11:122987018(-) A-I      intronic chr11:122972870(-) A-I      intronic chr11:122952407(-) A-I      intronic chr11:122953246(-) A-I      intronic chr11:122949633(-) A-I      intronic chr11:123007473(-) A-I      intronic chr11:123032289(-) A-I      intronic chr11:122951714(-) A-I      intronic chr11:122951173(-) A-I      intronic chr11:122990037(-) A-I      intronic chr11:123009636(-) A-I      intronic chr11:122995721(-) A-I      intronic chr11:122951208(-) A-I      intronic chr11:122947742(-) A-I      intronic chr11:122946698(-) A-I      intronic chr11:122973544(-) A-I      intronic chr11:122951200(-) A-I      intronic chr11:122973056(-) A-I      intronic chr11:122950278(-) A-I      intronic chr11:122964282(-) A-I      intronic chr11:123042301(-) A-I      intronic chr11:122946646(-) A-I      intronic chr11:122973182(-) A-I      intronic chr11:123003649(-) A-I      intronic chr11:122959875(-) A-I      intronic chr11:122948775(-) A-I      intronic chr11:122945861(-) A-I      intronic chr11:122951685(-) A-I      intronic chr11:122972635(-) A-I      intronic chr11:123014695(-) A-I      intronic chr11:122964904(-) A-I      intronic chr11:122945788(-) A-I      intronic chr11:122951778(-) A-I      intronic chr11:122972878(-) A-I      intronic chr11:122987275(-) A-I      intronic chr11:122953282(-) A-I      intronic chr11:122973162(-) A-I      intronic chr11:122953251(-) A-I      intronic chr11:122950267(-) A-I      intronic chr11:122999786(-) A-I      intronic chr11:122946737(-) A-I      intronic chr11:122991605(-) A-I      intronic chr11:122982048(-) A-I      intronic chr11:122956622(-) A-I      intronic chr11:122947884(-) A-I      intronic chr11:123004485(-) A-I      intronic chr11:122946781(-) A-I      intronic chr11:122952487(-) A-I      intronic chr11:122953281(-) A-I      intronic chr11:122948113(-) A-I      intronic chr11:123016996(-) A-I      intronic chr11:122951608(-) A-I      intronic chr11:122948270(-) A-I      intronic chr11:122952322(-) A-I      intronic chr11:122989956(-) A-I      intronic chr11:122943089(-) A-I      3'UTR chr11:122943051(-) A-I      3'UTR chr11:122943084(-) A-I      3'UTR chr11:122943047(-) A-I      3'UTR chr11:122943079(-) A-I      3'UTR

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	CLMP	chr11:122943085(-) A-I exon 3'UTR    15342557   //21960545 chr11:122946294(-) T-C intron -    22484847 chr11:122946302(-) T-C intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	CLMP	chr11:122940745-122940746(-) m6A 3'UTR       - chr11:122940762-122940763(-) m6A 3'UTR       - chr11:122940773-122940774(-) m6A 3'UTR       - chr11:122940827-122940828(-) m6A 3'UTR       - chr11:122940832-122940833(-) m6A 3'UTR       - chr11:122940864-122940865(-) m6A 3'UTR       - chr11:122940902-122940903(-) m6A 3'UTR       - chr11:122940939-122940940(-) m6A 3'UTR       - chr11:122940991-122940992(-) m6A 3'UTR       - chr11:122941070-122941071(-) m6A 3'UTR       - chr11:122941091-122941092(-) m6A 3'UTR       - chr11:122941125-122941126(-) m6A 3'UTR       - chr11:122941134-122941135(-) m6A 3'UTR       - chr11:122943368-122943369(-) m6A 3'UTR       24284625//24981863 chr11:122943428-122943429(-) m6A 3'UTR       24284625//24981863 chr11:122943496-122943497(-) m6A 3'UTR       24284625//24981863//22575960 chr11:122943657-122943658(-) m6A 3'UTR       24284625//24981863//22575960 chr11:122943672-122943673(-) m6A 3'UTR       24284625//24981863//22575960 chr11:122943729-122943730(-) m6A 3'UTR       24284625//24981863//22575960 chr11:122943742-122943743(-) m6A 3'UTR       24284625//24981863//22575960 chr11:122943767-122943768(-) m6A 3'UTR       24284625//24981863//22575960 chr11:122943862-122943863(-) m6A 3'UTR       24284625//25456834//24981863//22575960 chr11:122943908-122943909(-) m6A 3'UTR       24284625//25456834//24981863//22575960 chr11:122943954-122943955(-) m6A 3'UTR       24284625//25456834//24981863//22575960 chr11:122944012-122944013(-) m6A 3'UTR       24284625//25456834//24981863//22575960 chr11:122944049-122944050(-) m6A 3'UTR       24284625//25456834//24981863//22575960 chr11:122944131-122944132(-) m6A 3'UTR       24284625//24209618//25456834//24981863//22575960 chr11:122944170-122944171(-) m6A 3'UTR       24284625//24209618//25456834//24981863//22575960 chr11:122944231-122944232(-) m6A CDS//exon       24284625//24209618//25456834//24981863//22575960 chr11:122944271-122944272(-) m6A CDS//exon       24284625//25456834//24981863//27773536//22575960 chr11:122944354-122944355(-) m6A CDS//exon       24284625//25456834//24981863//27773536//22575960 chr11:122944445-122944446(-) m6A CDS//exon       24284625//24981863 chr11:122945423-122945424(-) m6A CDS//exon       24284625 chr11:122945451-122945452(-) m6A CDS//exon       24284625 chr11:122953859-122953860(-) m6A CDS//exon       22575960 chr11:122968624-122968625(-) Gm CDS//exon       - chr11:123065937-123065938(-) m6A 5'UTR       -

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	hsa-miR-4521	Exosome B cell lymphoma cell lines|EBV-transformed lymphoblastoid B cells     25242326 Exosome Breast milk|Cell lines|Epididymal epithelial cells|Osteoblast|Plasma|Primary glioblastoma neurosphere cells|Primary keratinocyte|Saliva|Serum|Urine     - Microvesicle Fibroblasts|Mesenchymal stem cells     -
	CLMP	Exosome Blood     - Nucleus HeLa-S3 cells     -

Interaction Network (The top 100 interactions):

Interactor1: hsa-miR-4521
Interactor2: CLMP

Evidence Support:

Weak-Evidence	RNA-Seq
Prediction-Evidence	miRDB//Targetscan
Support Database	ViRBase

References:

PMID	31570108	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	ARPE-19 human RPE cells	Interactor2 expression	None
Description	Table 1 List of miRNAs that were differentially expressed between EBOV- and mock-infected human RPE cells at 24 h post-infection.Putative target genes in EBOV-infected human RPE cells were predicted from the 15 highly differentially expressed miRNAs using algorithms: 2629 targets by Diana microT; 1799 targets by miRDB; and 14315 targets by TargetScan (Additional file 5).