| Detail: | |
| ncRNA Symmbol: | miR-145 |
|---|---|
| ncRNA Category: | miRNA |
| Interaction Gene Symbol: | RTKN |
| Death Pathway: | apoptosis |
| Expression Direction of ncRNA: | up |
| Species: | Homo sapiens |
| Tissue: | breast cancer cells |
| PMID: | 19360360 |
| Detail Description: | Herein, we show that miR-145 is down-regulated in human cancer cell line MCF-7 when compared to normal human mammary epithelial cell line MCF10A. Overexpression of miR-145 by plasmid inhibits MCF-7 cell growth and induces apoptosis. Subsequently, RTKN is identified as a potential miR-145 target by bioinformatics. Using reporter constructs, we show that the RTKN 3' untranslated region (3'UTR) carries the directly binding site of miR-145. Additionally, overexpression of miR-145 in MCF-7 reduces RTKN protein expression as well as mRNA level. Furthermore, down-regulation of RTKN by siRNA can inhibit MCF-7 cell growth. Taken together, we propose that loss of miR-145 may provide a selective growth advantage for MCF-7 by targeting RTKN. |
The 'First Node' or 'Second Node' option represents the sub-network of interacting entry with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting entry with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.