| Detail: | |
| ncRNA Symmbol: | miR-145 |
|---|---|
| ncRNA Category: | miRNA |
| Interaction Gene Symbol: | API5 |
| Death Pathway: | apoptosis |
| Expression Direction of ncRNA: | |
| Species: | Homo sapiens |
| Tissue: | colon cancer cell |
| PMID: | 25477374 |
| Detail Description: | Primary transcripts and mature miR-143 and miR-145 were quantified by real-time PCR, putative targets were measured by Western blotting.Putative targets of these miRNAs were assessed following transfection with miR-143 or miR-145. In agreement with increased expression of miR-143 and miR-145 in proximal colon, predicted targets of these miRNAs, apoptosis inhibitor 5 (API5), ERK5, K-RAS, and insulin receptor substrate 1 (IRS-1), which are cell cycle and survival regulators, were expressed at a lower level in proximal than distal colon. Transfection of HCA-7 colon cancer cells with miR-145 downregulated IRS-1, and transfection of HT-29 colon cancer cells with miR-143 decreased K-RAS and ERK5 expression. In conclusion, miR-143 and miR-145 and the predicted target proteins API5, ERK5, K-RAS, and IRS-1 display regional differences in expression in the colon. |
The 'First Node' or 'Second Node' option represents the sub-network of interacting entry with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting entry with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.