| Detail: | |
| ncRNA Symmbol: | miR-20a |
|---|---|
| ncRNA Category: | miRNA |
| Interaction Gene Symbol: | MAP1LC3B |
| Death Pathway: | autophagy |
| Expression Direction of ncRNA: | down |
| Species: | Homo sapiens |
| Tissue: | osteoclast |
| PMID: | 25485521 |
| Detail Description: | The results from dual luciferase reporter assay revealed that miR-20a directly targets Atg16l1 by binding to its 3'UTR end. Further, miR-20a transfection studies showed significant down regulation of autophagic proteins (LC3-II and ATG16L1) and osteoclast differentiation markers (Nfatc1, Traf6, and Trap) thus confirming the functional role of miR-20a under hypoxic conditions. Results of chromatin immunoprecipitation assay showed that HIF-1¦Á binds to miRNA-20a. From miRNA Q-PCR results, we confirmed that shRNA HIF-1¦Á knockdown significantly downregulated both autophagy (LC3, p62, Atg5, Atg12, Atg16l1, Atg7, Becn1, Atg9a) and osteoclast markers (Traf6, Nfatc1, Ctsk, cFos, Mmp9, Trap) in RAW264.7 cells. Thus, our findings suggest that the regulatory axis of HIF-1¦Á-miRNA-20a-Atg16l1 might be a critical mechanism for hypoxia-induced osteoclast differentiation. |
The 'First Node' or 'Second Node' option represents the sub-network of interacting entry with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting entry with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.