The mRNA was detected by transfection assay, western blotting, qRT-PCR, luciferase assay, siRNA and proliferation assay in SNB19 cell and SF188 cell, which can act as a decoy for Pten-targeting microRNAs.
These include 7,000 genes whose transcripts act as miR sponges and 148 genes that act through alternative, nonsponge interactions. Biochemical analyses in cell lines confirmed that this network regulates established drivers of tumor initiation and subtype implementation, including PTEN, PDGFRA, RB1, VEGFA, STAT3, and RUNX1, suggesting that these interactions mediate crosstalk between canonical oncogenic pathways. See Figure 5A for details.
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