The mRNA was detected by transfection assay, western blotting, qRT-PCR, luciferase assay,RNA-immunoprecipitation in DU145 cell, which can bind miR-17, miR-19a, miR-20a, miR-20b, miR-26b, miR-106a, miR-106b, acting as a decoy for PTEN-targeting microRNAs.
By a combined computational and experimental approach, we identified and validated endogenous protein-coding transcripts that regulate PTEN, antagonize PI3K/AKT signaling, and possess growth- and tumor-suppressive properties.
Go Back