The mRNA was detected by transfection assay, PCR identification for miRNA-UTR interaction and luciferase assay in 293T cell and DU145 cell, which can bind miR-17, miR-21, miR-93, miR-106b, miR-214, miR-19, miR-26, miR-19b, miR-20a, acting as a decoy for PTENP1-targeting microRNAs.
Given that microRNAs bind to RNAs, we hypothesized that RNAs could possess a regulatory role that relies on their ability to compete for microRNA binding. We also demonstrate that the transcripts of protein-coding genes such as PTEN are biologically active. These findings attribute a novel biological role to expressed pseudogenes, as they can regulate coding gene expression, and reveal a non-coding function for mRNAs.
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