Go Back

Detail Information


Basic information:


Translated ncRNA: circRNA

Related IDs cncRNA ID CNC_N_000006
Name circPde8
Gene ID 37741
Location Coordinate(Strand) 2R:23657567-23680844(+)
Locus Sequence
Organism Drosophila melanogaster

Evidence support:

  
In vivo/vitro assay In vitro assay
Low-throughput method GFP fluorescence
High-throughput method /
Tissue/Cell S2 cell
Description of experimental evidence The peptide was detected by GFP fluorescence expression analysis in S1 cell

Orthologues:

  
Human ENSG00000113231  
Chimpanzee ENSPTRG00000017010  
Mouse ENSMUSG00000021684  
Zebrafish ENSDARG00000063706  

References:

  
PMID 28344080
Description We focused on the ribo-circRNAs circMbl, circCdi, and circPde8 and used as negative controls circRNAs for which we did not observe RFP reads in this cell type: circHaspin and circCamK1. We engineered the minigenes to express a V5-tagged protein in case of a circularization event (Figure 2A). Cells transfected with circMblV5, circCdiV5, or circPde8V5 minigenes produced V5-tagged proteins, whereas those transfected with circHaspinV5 or circCamK1V5 minigenes failed to express a V5-tagged protein. we found a consistent and very high confidence hit for a peptide that can only be produced by circMbl3. This circRNA encodes a 37.04 kDa protein and might either include or skip an alternative internal exon.