Basic information:

Translated ncRNA: circRNA

Related IDs	cncRNA ID	CNC_N_000004
	Name	circMbl3
	Gene ID	36945
Location	Coordinate(Strand)	2R:17216549-17379376(+)
Locus Sequence
Organism	Drosophila melanogaster

Peptide information:

  

Peptide	RAADTTDMFPLIM
Peptide length	13

Evidence support:

  

In vivo/vitro assay	In vitro assay
Low-throughput method	GFP fluorescence;Western blotting;Selected reaction monitoring (SRM)
High-throughput method	/
Tissue/Cell	S2 cell;Head
Description of experimental evidence	The peptide was detected by western blotting and SRM in S2 cell, which was validated in S2 cell and head.

Orthologues:

  

Human	ENSG00000076770
Chimpanzee	ENSPTRG00000022280
Mouse	ENSMUSG00000036109
Zebrafish	ENSDARG00000016085

References:

  

PMID

28344080

Description

We focused on the ribo-circRNAs circMbl, circCdi, and circPde8 and used as negative controls circRNAs for which we did not observe RFP reads in this cell type: circHaspin and circCamK1. We engineered the minigenes to express a V5-tagged protein in case of a circularization event (Figure 2A). Cells transfected with circMblV5, circCdiV5, or circPde8V5 minigenes produced V5-tagged proteins, whereas those transfected with circHaspinV5 or circCamK1V5 minigenes failed to express a V5-tagged protein. we found a consistent and very high confidence hit for a peptide that can only be produced by circMbl3. This circRNA encodes a 37.04 kDa protein and might either include or skip an alternative internal exon.