Detail

Basic information:

Translated ncRNA: circRNA

Related IDs	cncRNA ID	CNC_N_000001
	Name	circCdi
	Gene ID	42289
Location	Coordinate(Strand)	3R:19044882-19094270(-)
Locus Sequence
Organism	Drosophila melanogaster

Evidence support:

In vivo/vitro assay	In vitro assay
Low-throughput method	GFP fluorescence
High-throughput method	/
Tissue/Cell	S2 cell
Description of experimental evidence	The peptide was detected by GFP fluorescence expression analysis in S2 cell

Orthologues:

Human	/
Chimpanzee	/
Mouse	/
Zebrafish	/

References:

PMID	28344080
Description	We focused on the ribo-circRNAs circMbl, circCdi, and circPde8 and used as negative controls circRNAs for which we did not observe RFP reads in this cell type: circHaspin and circCamK1. We engineered the minigenes to express a V5-tagged protein in case of a circularization event (Figure 2A). Cells transfected with circMblV5, circCdiV5, or circPde8V5 minigenes produced V5-tagged proteins, whereas those transfected with circHaspinV5 or circCamK1V5 minigenes failed to express a V5-tagged protein. we found a consistent and very high confidence hit for a peptide that can only be produced by circMbl3. This circRNA encodes a 37.04 kDa protein and might either include or skip an alternative internal exon.

PMID

28344080

Description

We focused on the ribo-circRNAs circMbl, circCdi, and circPde8 and used as negative controls circRNAs for which we did not observe RFP reads in this cell type: circHaspin and circCamK1. We engineered the minigenes to express a V5-tagged protein in case of a circularization event (Figure 2A). Cells transfected with circMblV5, circCdiV5, or circPde8V5 minigenes produced V5-tagged proteins, whereas those transfected with circHaspinV5 or circCamK1V5 minigenes failed to express a V5-tagged protein. we found a consistent and very high confidence hit for a peptide that can only be produced by circMbl3. This circRNA encodes a 37.04 kDa protein and might either include or skip an alternative internal exon.